the effect of migri-heal® on nitric oxide production in an in vitro inflammatory model of primary microglial cells

background: recently, much attention has been directed towards considering activated microgelial cells  as putative  targets for treatment of neurological disorders. migriheal®  as a novel herbal remedy was introduced   for  the treatment of  migraine headaches.  the previous researches has shown that migriheal® extracts can decrease no in  an in vitro inflammatory model. the aim of this study was to investigate the effect of migriheal® on no generation from lps- stimulated microglia cells. materials and methods: neonatal rat primary microglial cells were isolated from the mixed glial cultures and the purity of the cultures was determined by immunocytochemistry. microglial cells were pretreated with migri-heal® and activated by 1μg/ml lps. subsequently, no levels in the culture supernatants were measured by a griess reaction. our results showed that migri-heal® 50μg/ml significantly reduced no level in inflamed microglia in a dose-dependent manner.   results: the results showed that different  concentrations of migri-heal® had no prominent effect on cell viability in presence of lps as compared with the control group.  in addition, the pretreatment of microglia cells with migri-heal®  can prevent from a morphological changes of the cells into the round and phagocytic shape. conclusion: our study demonstrated that migriheal® might have no scavenging properties. integrative studies are warranted to uncover the   novel pharmacological insights of this herbal remedy as an putative   therapeutic approach  against diseases - associated with inflammation. k e y w o r ds: inflammation, microglia, migri-heal®, nitric oxide